The BioRad Radiance 2100MP Multiphoton System is attached to an Olympus fixed-stage upright microscope (BX51WI) and is designed to facilitate patch clamp recording. The illumination sources are the Spectra-Physics MaiTai multi-photon IR laser and a 150 watt Xe arc lamp for widefield imaging. This system also includes all the equipment necessary to do patch clamp recording in living preparations as well as the Becker and Hinkel lifetime (FLIM) system with a microchannel plate PMT detector. Currently this system can only accommodate brain slices or cultured cells on small coverslips. In the future the microscope stage will be modified to accomodate specimens on conventional microscope slides. This change will greatly expand the user base for this instrument which is currently restricted to the major user group specified in the grant proposal.

• Dr. Ben Strowbridge
  Associate Professor
  x 6974
  bxs48@po.cwru.edu

• Maryanne Pendergast
  Training and Facility Manager
  x 2575
  mxp19@po.cwru.edu